Part:BBa_K2334002:Design

J23100 + RiboJ + B0034 + pucM, HIU hydrolase generator

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 395
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 279
  

Design Notes

In our project, PucM is used as the important enzyme in urate degradation, whose expression is driven by J23100 in this part. RiboJ is used for the quantitive pathway construction.

Source

Bacillus Sublitis genomic